granulysin  (R&D Systems)

Journal: Revista da Associação Médica Brasileira

Article Title: Assessment of serum granulysin and cathepsin-L levels in vitiligo patients

doi: 10.1590/1806-9282.20231107

Figure Lengend Snippet: Serum granulysin and cathepsin-L levels of the patient and control groups.

Article Snippet: For the measurement of granulysin in serum, the BT LAB brand ELISA kit for human granulysin was used with a reference range of 0.5–100 ng/mL; for the measurement of cathepsin-L in serum, the BT LAB brand ELISA kit for human cathepsin-L was used with a reference range of 0.1–40 ng/mL.

Techniques: Control

Journal: Revista da Associação Médica Brasileira

Article Title: Assessment of serum granulysin and cathepsin-L levels in vitiligo patients

doi: 10.1590/1806-9282.20231107

Figure Lengend Snippet: Correlation between serum granulysin and cathepsin-L levels in the patient group.

Article Snippet: For the measurement of granulysin in serum, the BT LAB brand ELISA kit for human granulysin was used with a reference range of 0.5–100 ng/mL; for the measurement of cathepsin-L in serum, the BT LAB brand ELISA kit for human cathepsin-L was used with a reference range of 0.1–40 ng/mL.

Techniques:

Journal: bioRxiv

Article Title: Blinatumomab-driven T-cell activation in αβ and γδ T-cell subsets: Insights from in vitro assays§

doi: 10.1101/2025.11.17.688178

Figure Lengend Snippet: A: BCP-ALL cells in the presence (red) or absence (grey) of BLN for three days were co-cultured with freshly isolated γδ, CD4⁺ and CD8⁺ αβ T cells/ T- and B-cell frequencies in the coculture were assessed by staining for CD3 and CD19, as exemplary shown for CD8⁺ αβ T cells. B: The release of IFNγ, GrzB and Prf into supernatants of cocultures in the presence (red) or absence (grey) of BLN was assessed via ELISA after three days, and the release of Gnly was analyzed after seven days. Data includes 4 to 7 healthy donors and * indicates p value between 0.01-0.05, ** indicates p value between 0.01-0.001 (paired and unpaired T-test).

Article Snippet: Cell culture supernatants were analyzed for Interferon-γ (IFNγ; DIF50C), Granzyme B (GrzB; DGZB00), Perforin (QK8011), Granulysin (Gnly; DY3138), Perforin (Prf; QK8011) and soluble Fas-Ligand (sFAS-L; DFL00B) using sandwich ELISA kits from R&D Systems, Minneapolis, USA according to the manufacturer’s instructions.

Techniques: Cell Culture, Isolation, Staining, Enzyme-linked Immunosorbent Assay

Journal: Revista da Associação Médica Brasileira

Article Title: Assessment of serum granulysin and cathepsin-L levels in vitiligo patients

doi: 10.1590/1806-9282.20231107

Figure Lengend Snippet: Serum granulysin and cathepsin-L levels of the patient and control groups.

Article Snippet: For the measurement of granulysin in serum, the BT LAB brand ELISA kit for human granulysin was used with a reference range of 0.5–100 ng/mL; for the measurement of cathepsin-L in serum, the BT LAB brand ELISA kit for human cathepsin-L was used with a reference range of 0.1–40 ng/mL.

Techniques: Control

Journal: Revista da Associação Médica Brasileira

Article Title: Assessment of serum granulysin and cathepsin-L levels in vitiligo patients

doi: 10.1590/1806-9282.20231107

Figure Lengend Snippet: Correlation between serum granulysin and cathepsin-L levels in the patient group.

Article Snippet: For the measurement of granulysin in serum, the BT LAB brand ELISA kit for human granulysin was used with a reference range of 0.5–100 ng/mL; for the measurement of cathepsin-L in serum, the BT LAB brand ELISA kit for human cathepsin-L was used with a reference range of 0.1–40 ng/mL.

Techniques:

Journal: Ecotoxicology and environmental safety

Article Title: Granulysin-mediated reduction of PDZRN3 induces Cx43 gap junctions activity exacerbating skin damage in trichloroethylene hypersensitivity syndrome.

doi: 10.1016/j.ecoenv.2024.116174

Figure Lengend Snippet: Fig. 2. GNLY released from TCE-activated CD3-CD56+NK cells or CD3+CD56+NKT cells promote apoptosis. (A) GNLY expression in PBMCs from health donors after treatment of TCE (1 μM) or chloral hydrate (1 μM) for 7 days by flow cytometry. (B) ELISA analysis for GNLY expression in PBMCs supernatant after treatment with TCE (1 μM) or chloral hydrate (1 μM) on day1, 3 and 5 (n=7). (C) Western blot analysis for the expression of GNLY in PBMCs. (D) The extent of apoptosis in HaCaT cells treated with supernatants for 48 h derived from PBMCs stimulated with TCE or chloral hydrate for 7 days was assessed using flow cytometry (n=3). (E) The expressions of apoptosis-related genes in HaCaT cells under GNLY(+)SNF(TCE) or GNLY(+)SNF(chloral hydrate) for 24 or 48 hours were detected by qPCR (n=3). Color scale ranges from red to green which respectively denotes the up- or down-regulated of the genes. (F) The expression of TNFRSF1B, IRE1, CHOP genes, and Bax/ Bcl2 ratio in HaCaT cells under different treatments, such as DMSO (solvent control), GNLY(+)SNF(TCE), GNLY(+)SNF(chloral hydrate), GNLY(Nab)SNF(TCE), GNLY(Nab)SNF(chloral

Article Snippet: The human GNLY ELISA kit was purchased from Arigo (Wu Han, China).

Techniques: Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Western Blot, Derivative Assay, Solvent, Control

Journal: Ecotoxicology and environmental safety

Article Title: Granulysin-mediated reduction of PDZRN3 induces Cx43 gap junctions activity exacerbating skin damage in trichloroethylene hypersensitivity syndrome.

doi: 10.1016/j.ecoenv.2024.116174

Figure Lengend Snippet: Fig. 3. GNLY could exacerbate skin damage in the THS mouse model. (A) Histopathological changes in the skin (Scale bars, 100 μm). Orange arrows represent epidermal cuticle significant parakeratosis and epidermal cell layer thickening. Green arrow represented neutrophils. Blue arrow represented leukomonocyte. Red circle represented hyperemia. (B) Leukocyte types was used by hematology analyzer (n=5). * indicated P < 0.05, vs control group. (C) The percentage of the CD3+CD8+ T cells and CD3-CD56+NK cells in spleen samples were detected by flow cytometry (n=5). * indicated P < 0.05, vs control group. # indicated P < 0.05, vs TCE group. (D) The level of cells apoptosis in the skin by TUNEL Apoptosis Assay kit (Scale bars, 100 μm). Nuclei were stained with DAPI (blue), apoptosis cells were stained with TUNEL (green). (E) Western blot analysis for the expression of apoptosis-related proteins (TNFRSF1B, cleaved caspase3, Bcl2 and Bax), inflammation- related proteins (P-p65, p65, NLRP3, ASC and cleaved caspase1) and Cx43 protein in skin tissue.

Article Snippet: The human GNLY ELISA kit was purchased from Arigo (Wu Han, China).

Techniques: Control, Flow Cytometry, TUNEL Assay, Apoptosis Assay, Staining, Western Blot, Expressing

Journal: Ecotoxicology and environmental safety

Article Title: Granulysin-mediated reduction of PDZRN3 induces Cx43 gap junctions activity exacerbating skin damage in trichloroethylene hypersensitivity syndrome.

doi: 10.1016/j.ecoenv.2024.116174

Figure Lengend Snippet: Fig. 4. GNLY could induce the cellular communication disorders, inflammation and apoptosis in HaCaT cells. (A) The CCK-8 assay was used to evaluate the viability of HaCaT cells after exposure to different concentrations of GNLY for 12, 24, and 48 hours. (B) Western blot analysis for the expression of inflammation-related proteins (P-p65, p65, NLRP3, ASC and cleaved caspase1) in HaCaT cells. (C) The extent of apoptosis in cells was assessed by flow cytometry. (D) The Western blot analysis measured the expression of apoptosis-related proteins, including TNFRSF1B, cleaved caspase3, Bcl2, and Bax. (E) Representative immunofluorescent images of HaCaT cells. Nuclei were mrked blue and Cx43 protein were marked green (Scale bars, 100 μm). (F) The level of gap junction activity was detected by immunofluorescence method (Scale bars, 50 μm). Lucifer yellow (LY, green) was transferred to neighboring cells through open gap junctions. Bar graphs show the means ± standard (n=3). * indicated P < 0.05, ** indicated P < 0.01, vs control group.

Article Snippet: The human GNLY ELISA kit was purchased from Arigo (Wu Han, China).

Techniques: CCK-8 Assay, Western Blot, Expressing, Flow Cytometry, Activity Assay, Immunofluorescence, Control

Journal: Ecotoxicology and environmental safety

Article Title: Granulysin-mediated reduction of PDZRN3 induces Cx43 gap junctions activity exacerbating skin damage in trichloroethylene hypersensitivity syndrome.

doi: 10.1016/j.ecoenv.2024.116174

Figure Lengend Snippet: Fig. 6. Targeting the E3 ubiquitin-protein ligase PDZRN3 regulates cell apoptosis and inflammation. Before GNLY (300 ng/mL) treatment, cells were transfected with siRNA-PDZRN3 or plasmid-PDZRN3 for 48 h to down-regulate or up-regulate PDZRN3 expression. (A) Protein expression of Cx43 was detected by immuno fluorescence. Nuclei were stained blue and Cx43 protein was stained green (scale bars, 100 μm). (B) The level of gap junction activity was detected by immuno fluorescence (scale bars, 50 μm). Lucifer yellow (LY, green) was transferred to neighboring cells through open gap junctions. (C) Flow cytometric analysis of apoptosis level in HaCaT cells. (D) Western blot for the expression of Cx43 protein, apoptosis-related proteins and inflammation-related proteins in HaCaT cells. Bar graphs show the mean ± standard (n=3). * indicated P < 0.05. ** indicated P < 0.01, vs negative control group. # ↑indicated P < 0.05, vs GNLY group. ↓ indicated down- regulate of PDZRN3 using siRNA-PDZRN3. indicated up-regulate of PDZRN3 using plasmid-PDZRN3.

Article Snippet: The human GNLY ELISA kit was purchased from Arigo (Wu Han, China).

Techniques: Ubiquitin Proteomics, Transfection, Plasmid Preparation, Expressing, Fluorescence, Staining, Activity Assay, Western Blot, Negative Control

Journal: Ecotoxicology and environmental safety

Article Title: Granulysin-mediated reduction of PDZRN3 induces Cx43 gap junctions activity exacerbating skin damage in trichloroethylene hypersensitivity syndrome.

doi: 10.1016/j.ecoenv.2024.116174

Figure Lengend Snippet: Fig. 5. GNLY released from TCE-activated NK cells or synthesized GNLY could both induce dysregulation of cell auto-ubiquitination. (A) The auto-ubiquitylation activity in HacaT cells stimulated with GNLY or GNLY(+)SNF(TCE) for 6 h was detected by the E3 ligase auto-ubiquitylation assay kit. (B) PCR Array analysis of apoptosis genes after exposure to GNLY(a) or GNLY(+)SNF(TCE) (b) in HaCaT cells, showing mean -log10 adjusted P values. Downregulated genes with significantly different P values are shown in green, non-significantly regulated genes in gray, and upregulated genes with significantly different P values in red. PCR Array analysis was performed for three independent experiments per condition.

Article Snippet: The human GNLY ELISA kit was purchased from Arigo (Wu Han, China).

Techniques: Synthesized, Ubiquitin Proteomics, Activity Assay, Ubiquitin Assay